diff --git a/tests/functional/nix-shell.sh b/tests/functional/nix-shell.sh
index edaa1249b..13403fadb 100644
--- a/tests/functional/nix-shell.sh
+++ b/tests/functional/nix-shell.sh
@@ -84,6 +84,11 @@ chmod a+rx $TEST_ROOT/spaced\ \\\'\"shell.shebang.rb
 output=$($TEST_ROOT/spaced\ \\\'\"shell.shebang.rb abc ruby)
 [ "$output" = '-e load(ARGV.shift) -- '"$TEST_ROOT"'/spaced \'\''"shell.shebang.rb abc ruby' ]
 
+# Test nix-shell shebang quoting
+sed -e "s|@ENV_PROG@|$(type -P env)|" shell.shebang.nix > $TEST_ROOT/shell.shebang.nix
+chmod a+rx $TEST_ROOT/shell.shebang.nix
+$TEST_ROOT/shell.shebang.nix
+
 # Test 'nix develop'.
 nix develop -f "$shellDotNix" shellDrv -c bash -c '[[ -n $stdenv ]]'
 
diff --git a/tests/functional/shell.shebang.nix b/tests/functional/shell.shebang.nix
new file mode 100755
index 000000000..08e43d53c
--- /dev/null
+++ b/tests/functional/shell.shebang.nix
@@ -0,0 +1,10 @@
+#! @ENV_PROG@ nix-shell
+#! nix-shell -I nixpkgs=shell.nix --no-substitute
+#! nix-shell    --argstr s1 'foo "bar" \baz'"'"'qux'  --argstr s2 "foo 'bar' \"\baz" --argstr s3 \foo\ bar\'baz --argstr s4 ''  
+#! nix-shell shell.shebang.nix --command true
+{ s1, s2, s3, s4 }:
+assert s1 == ''foo "bar" \baz'qux'';
+assert s2 == "foo 'bar' \"baz";
+assert s3 == "foo bar'baz";
+assert s4 == "";
+(import <nixpkgs> {}).runCommand "nix-shell" {} ""